indirect evidence for the presence of ribonucleic acid in vesicular stomatitis virions

Indirect evidence for the presence of Ribonucleic acid in vesicular stomatitis Virions

Indirect evidence for the presence of ribonucleic acid in vesicular stomatitis virions.

Concentration of 5 gamma g(ml or less of 5-Auorodeoxyuridine (FDC) and of 5-bromodeoxyridine (BDU) inhibited the growth of adenovirus type 5 and of vaccinia virus in ERK and chick ell1bryo cells, provided that adequate time was allowed for the inhibitors to act. With the same proviso, FD {T and BDU also inhibited the growth of ERK cells. Cnder similar conditions of cultivation, concentrations o...

Ribonucleic acid polymerase in virions of Newcastle disease virus: comparison with the vesicular stomatitis virus polymerase.

The virions of Newcastle disease virus (NDV) contained an enzyme that catalyzed the incorporation of ribonucleotides into ribonucleic acid (RNA). Optimal conditions for this polymerase activity were identical to the conditions for the vesicular stomatitis virus (VSV) polymerase, and both enzymes were active for longer times at 32 C than at 37 C. However, the specific activity of the NDV polymer...

Adenylate-rich sequences in vesicular stomatitis virus messenger ribonucleic acid.

Vesicular stomatitis virus (VSV)-specific messenger ribonucleic acid (mRNA) species contain sequences of adenylate-rich RNA which are more heterogeneous in their migration through sodium dodecyl sulfate-polyacrylamide gels than the corresponding fractions from HeLa cell mRNA. VSV virion RNA contains no adenylaterich sequences. The possible role of such sequences in the mRNA species of a cytopla...

Ribonucleic acid synthesis of vesicular stomatitis virus, II. An RNA polymerase in the virion.

The virions of vesicular stomatitis virus contain an enzyme that catalyzes the incorporation of ribonucleotides into RNA. The product of the reaction is mainly RNA complementary in base sequence to that of vesicular stomatitis virus RNA.

Induced ribonucleic acids in cells infected with vesicular stomatitis virus.

RNA molecules labelled in BHK 2I cells following infection with vesicular stomatitis virus in the presence of Actinomycin D and 32PO 4 were examined. A complex pattern was obtained when the RN A was centrifuged in sucrose gradients, at least five radioactive peaks being found. These had sedimentation coefficients in sucrose gradients in o-I M-sodium acetate, pH 5.o, of approx. 38 S, 28 S, 20 S,...